Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 25;37(11):2114–2127. doi: 10.1161/ATVBAHA.117.309852

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited and is not used for commercial purposes.

PMC Copyright notice

Figure 5. — The expression of leptin was upregulated after surgery both in the circulation and in the injured artery. A, Serum leptin in wild-type mice 1 (n=10), 7 (n=5) and 14 (n=12) d after injury were quantified by using leptin ELISA kit. B, Serum leptin in wild-type and db/db mice was quantified by using leptin ELISA kit. C, Expression of leptin in the injured or noninjured arteries was documented by performing Western blotting 1 d post-surgery (n=4). D, Quantification of leptin expression in noninjured and injured vessels. E and F, Difference in expression of leptin (Alexa 488; green) in noninjured (E) artery (n=6) and injured (F) artery (n=10) was analyzed on 1 d post-surgery by immunofluorescence (scale bars, 30 μm). G, Expression of leptin in smooth muscle cell (Alexa 594; red) in vitro was detected by immunofluorescence (scale bars, 8 μm). DAPI indicates 4’,6-diamidino-2-phenylindole for nucleus staining; and EC, endothelium. All graphs are shown as mean±SEM. *P<0.05, **P<0.01, ***P<0.001.