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The expression of osteogenesis-related genes was significantly upregulated. After culture with conditioned medium, the relative expressions of differentiation markers Runx2, ALP, and Osterix in MG-63 cells were upregulated. Gene expression was detected by qRT-PCR. The expression of proliferation marker cyclin-D1 was also upregulated. Expression of these genes after 40 minutes of LIPUS treatment was significantly higher than after 20 minutes of LIPUS treatment.
