Position of transposons for all mla deficient strains are denoted by black arrows (A). qPCR of transcript levels for vacJ, normalized to 16S rRNA and Wt levels (B). LL-37 MIC for P. aeruginosa wild-type, mla deficient mutants, and vacJ− complemented strains. MIC results were performed 3 times (C). LL-37 killing kinetics of P. aeruginosa WT, vacJ−, and vacJ− complemented strains at 16 µM (D) and 8 µM (E). ** = P < 0.01 t-test.