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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Biol Psychiatry. 2017 May 5;82(11):794–805. doi: 10.1016/j.biopsych.2017.04.013

Figure 1.

Figure 1

Auts2 and Caln1 gene interactions are reduced by repeated cocaine administration. a, Number of detected fragments and number of counts per fragment plotted for chromosome location over the entire rat genome. Auts2 is located on chr12. Sal, saline; Coc, cocaine. b, Average counts per fragment on chr12. The 4C bait of Auts2 is located at 27,155 kbp. c, Pie chart of genomic locations of detected 4C fragments (shell and core combined). d, Venn diagram of significantly changed fragments in NAc shell and core. e, Overview over gene locations in mouse (chr5) and rat (chr12). f, 3C libraries were prepared from NAc shell punches of rats that had been I.P.-injected for 7 days with saline or 20 mg/kg cocaine and were analyzed 24 h later. N=3,3. Two-way ANOVA: interaction: F(11,48)=2.10, P<0.05; drug factor: F(1,48)=16.20, P<0.001; dilution factor: F(11,48)=6.56, P<0.0001. Bonferroni: sal vs. coc: **P<0.01; all following: P>0.05. g, 3C libraries were prepared from NAc core punches of rats that have acquired cocaine during self-administration for at least 7 days with 20 mg/kg cocaine. They were analyzed 24 h later. N=3,3. Two-way ANOVA: interaction: F(9,40)=11.40, P<0.0001; drug factor: F(1,40)=45.92, P<0.0001; dilution factor: F(9,40)=28.43, P<0.0001. Bonferroni: sal vs. coc: ***P<0.001; ***P<0.001; **P<0.01; all following: P>0.05. h, 3C libraries were prepared from NAc shell punches of rats that had been I.P.-injected for 7 days with saline or 20 mg/kg of cocaine and were analyzed 24 h later. N=3,3. Two-way ANOVA: interaction: F(11,48)=9.834, P<0.0001; drug factor: F(1,48)=31.34, P<0.0001; dilution factor: F(11,48)=35.06, P<0.0001. Bonferroni: sal vs. coc: ***P<0.001; ***P<0.001; all following: P>0.05. f-h, Average +/− SD shown.