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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Biol Psychiatry. 2017 May 5;82(11):794–805. doi: 10.1016/j.biopsych.2017.04.013

Figure 5.

Figure 5

D2-specific overexpression of Auts2 or Caln1 in NAc increases cocaine place conditioning. a, Procedure for cocaine conditioned place preference (CPP): On day 1, D1- or D2-Cre mice were subjected to 30 min pretest in the CPP chambers to balance for bias in preference. (On average, we use an unbiased procedure, where there is no overall preference for one side or the other.) On day 2, mice were stereotaxically injected into NAc with Auts2-p1005-LSIL, Caln1-p1005-LSIL or p1005-LSIL control HSV vectors (see photos - viral expression: green; DAPI: blue; magnifications clockwise: 10x, 20x, and 100x with 2x zoom). On days 4 and 5, mice were conditioned and on day 6, place preference was tested. b, CPP of D1-Cre mice. N=12,9,15. One-way ANOVA: F(5,71)=7.02, P<0.0001. Tukey’s test: pretest vs. test: GFP *P<0.05; Auts2: *P<0.05; Caln1: **P<0.01; gene factor within test: P>0.05. c, CPP of D2-Cre mice. N=8,14,9. One-way ANOVA: F(5,61)=9.36, P<0.0001. Tukey’s test: pretest vs. test: GFP ***P<0.001; Auts2: ***P<0.0001 ; Caln1: **P<0.01 ; within test: GFP vs. Auts2: *P<0.05; GFP vs. Caln1: *P<0.05. B,C, Average +/− SD shown.