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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Cardiovasc Electrophysiol. 2017 Jun 23;28(9):1070–1082. doi: 10.1111/jce.13259

Figure 1. Impact of hERG channel protein additions on protein expression and trafficking.

Figure 1

A) Cartoon graphic of a hERG channel subunit demonstrating the 4 variations discussed in this manuscript. B) Immunoblot of WT hERG, along with the inadvertent variation V198E/P202L, the N-terminal myc-tag and C-terminal myc-tag. C) Summary data for total hERG protein expression for each of the 3 variations shown in B. V198E/P202L has increased protein expression relative to WT hERG (n=4, p= 0.0182), the N-terminal myc fusion tag increased protein expression relative to WT hERG (n=4, p=0.0002), and the C-terminal myc fusion tag does not impact protein expression relative to WT hERG (n=5). D) Summary data for mature/immature ratio (155kDa/135kDa), or trafficking, for the 3 variants relative to WT hERG. V198E/P202L hERG has decreased trafficking efficiency (n=4, p=0.0002), N-Myc has increased trafficking efficiency relative to WT hERG (n=4, p= 0.0076) and C-Myc has decreased mature/immature ratio relative to WT hERG (n=5, p=0.0165)