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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Cardiovasc Electrophysiol. 2017 Jun 23;28(9):1070–1082. doi: 10.1111/jce.13259

Figure 2. The amino terminal myc-tag and the V198E/P202L double mutation increase hERG channel stability.

Figure 2

A) Pulse chase in-gel fluorescence scan of the WT hERG, V198E/P202L hERG, N-terminal-myc WT hERG, and N-terminal-myc V198E/P202L hERG expressed in HEK293T cells. Assay is representative of data determined from 4 independent experiments. B) Analysis of pulse chase data from 4 experiments. For each assay the 100% total protein value (immature and mature hERG protein) was defined by the densitometry at chase time 0 hours. The data points represent the mean of the experiments with the standard error of the mean (S.E.M.) displayed as error bars. C) the half-life values for hERG variants from panel B. The 95% confidence intervals are shown in parenthesis.