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PD-1/PD-L1 blockade enhances the pAKT signaling pathway in NK92 and NKL cells. (a and b) After pre-incubation with 2 μg/ml IgG or matched PD-1/PD-L1 blocking antibodies (aPD-1 or aPD-L1), NK92 or NKL cells were stimulated with PMA and ionomycin (a) or EC9706 target cells for 1 h, and western blot analysis was used to determine AKT phosphorylation. The results in a and b are representative of three independent experiments.
