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. 2017 Nov 1;8:1457. doi: 10.3389/fimmu.2017.01457

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Copyright © 2017 Li, Wang, Li, Yu and Xiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Yeast two-hybrid analysis. Yeast cells were transformed with a combination of the indicated plasmids. (A) (1) pGBK-p53 and pGAD-T antigen for positive control; (2) pGAD-VF1 and pGBK-VR2(1–4) plasmids; (3) pGAD-VF1 and pGBKT7 plasmids; (4) pGBK-VR2(1–4) and pGADT7 plasmids; (5) pGBK-Lam and pGAD-T antigen for negative control. (B) (1) pGBK-p53 and pGAD-T antigen for positive control; (2) pGAD-VF2 and pGBK-VR2(1–4) plasmids; (3) pGAD-VF2 and pGBKT7 plasmids; (4) pGBK-VR2(1–4) and pGADT7 plasmids; (5) pGBK-Lam and pGAD-T antigen for negative control. (C) (1) pGBK-p53 and pGAD-T antigen for positive control; (2) pGAD-VF3 and pGBK-VR2(1–4) plasmids; (3) pGAD-VF3 and pGBKT7 plasmids; (4) pGBK-VR2(1–4) and pGADT7 plasmids; (5) pGBK-Lam and pGAD-T antigen for negative control.