Table 1.
Information of primer sequences used in the present study.
| Primer name | Primer sequence (5′-3′) | Expected size (bp) | Annealing temperature (°C) | Primer sites (bp) |
|---|---|---|---|---|
| LvVEGFR2-F1 | GCAAGGTATTTATCCCAGTT | 1,956 | 56 | 145 |
| LvVEGFR2-R1 | CTTTAGGGTTAGGTTCTCAT | 2,100 | ||
| LvVEGFR2-F2 | CGATTATGAGAACCTAACCCT | 1,800 | 57 | 2,076 |
| LvVEGFR2-R2 | TCCTGAAGCCACAGCGATT | 3,875 | ||
| 18S-F | TATACGCTAGTGGAGCTGGAA | 136 | 56 | – |
| 18S-R | GGGGAGGTAGTGACGAAAAAT | – | ||
| LvVEGFR2-qF | TGCTCCTCGCAGACAACAAT | 188 | 57 | 3,293 |
| LvVEGFR2-qR | CCACAGAGTCACTCCAAAGG | 3,480 | ||
| VP28-qF | AAACCTCCGCATTCCTGTGA | 141 | 55 | – |
| VP28-qR | TCCGCATCTTCTTCCTTCAT | – | ||
| LvVEGFR2-dsF | TAATACGACTCACTATAGGG | 701 | 59 | 2,739 |
| GGCTTACTTGGGCAAACAT | ||||
| LvVEGFR2-dsR | TAATACGACTCACTATAGGG | 3,439 | ||
| TGAAGAAACGGTCACGAAT | ||||
| dsEGFP-F | TAATACGACTCACTATAGGG | 289 | 59 | – |
| CAGTGCTTCAGCCGCTACCC | ||||
| dsEGFP-R | TAATACGACTCACTATAGGG | – | ||
| AGTTCACCTTGATGCCGTTCTT | ||||
| LvVEGF1-pF | CCAAGATCTCTTTAACCCGATCTTCAGGG | 848 | 55 | – |
| LvVEGF1-pR | ACCAAGCTTTCAGAATAGCAAGGGTACAC | – | ||
| LvVEGF2-pF | CCAAGATCTGACCTTCGTTTATCCTGCCG | 931 | 58 | – |
| LvVEGF2-pR | ACCAAGCTTTTACGCGGGGCTACCTTCTG | – | ||
| LvVEGF3-pF | CCAAGATCTGAGGGGGTGCTTAAGCATTC | 650 | 55 | – |
| LvVEGF3-pR | ACCAAGCTTTCAGTCCATGCACCTGCATG | – | ||
| LvVEGFR2-pF | TTCAACCCAGTGCATGTTGAGGAG | 2,061 | 55 | 817 |
| LvVEGFR2-pR | CACAACTACTAGCCTCAGTTTGCG | 2,289 | ||
| LvFAK-qF | ATTACTCAACACCAGCAACC | 172 | 57 | – |
| LvFAK-qR | GTTCCCTCGGACTCCACCTT | – | ||
| LvPI3K-qF | TATGAAGTAACCCGTAGTGCCA | 187 | 57 | – |
| LvPI3K-qR | TGCCCACATCTCCTGACTGA | – |