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. 2017 Oct 20;6(10):e159. doi: 10.1038/cti.2017.41

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Copyright © 2017 The Author(s)

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(a) Immunophenotyping, proliferation and isotype switching in TCF3/ TNFRSF13B/TACI mutant B cells. (a) Immunophenotyping results indicating proportions of naïve (CD20⁺CD27⁻) and memory (CD20⁺CD27⁺) B cells, and CD4⁺ and CD8⁺ T cells in PBMCs isolated from available family members as indicated, and representative healthy donor controls. (b) Relative proportions of IgM/G/A memory B cells from each family member and unrelated healthy donors (each as a proportion of total memory B cells). IgM-expressing cells are shown in black, IgG⁻ in gray and IgA⁻ isotype switched memory B cells in white, as indicated. (c) Total numbers of lymphocytes, B cells, naïve (CD20⁺CD27⁻) and memory (CD20⁺CD27⁺) B cells in peripheral blood from each family member and unrelated healthy donors (HD=12). Immunophenotyping and cell counts were performed in two separate experiments.