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Hypoxia induces EMT. A, WB of HIF-1α, SOX2, and Claudin1 protein expression in hypoxic U87 and U251 cells. β-actin was used as the loading control. B, qRT-PCR analysis of SOX2, SNAI1 and ZEB1 at d5 under hypoxia. *p < 0.05, **p < 0.01, ***p < 0.001. C, WB of UGT8, MKP2, and RelA/p65 protein expression in hypoxic cells. β-actin was used as the loading control.
