Fig. 3.
Impact of Vorinosat on the expression level of proteins involved in protein folding pathways in NPC1I1061T fibroblasts. A–G, Western blot analysis of cell lysates from DMSO or Vorinostat treated NPC1I1061T fibroblasts. Cell lysates were resolved in SDS-PAGE and Western blotting with the indicated antibodies as described in Methods. In Vorinostat-treated NPC1I1061T fibroblasts FKBP10 (A), SERPINH1 (B), BAG2 (C), and GALNT5 (F) were downregulated whereas ERP44 (D), USP15 (E), and TRAP1 (G) were upregulated. ***p < 0.001, **p < 0.01, *p < 0.05, and n.s., not significant (p > 0.05). Bar graph represents the relative amount of the indicated protein normalized to the loading control GADPH or tubulin from three independent experiments.