Fig. 2.

Generation of candidate glycan list for the standard N-glycan GlyDB library. A, Seven seed glycans were provided as inputs for the “connection inference” program. B, Glycosylating enzymes used for pathway synthesis. Detailed enzyme rules and properties are defined in supplemental Table S2. C, Network diagram generated using one pair of input seed glycans (glycans i and v in panel A). D, Categorization of all glycans in panel c pathway into 19 groups using structure-based isomer classification. One representative glycan from each group is a member of the glycan database, GlyDB. Full network using all seed glycans and enzymes are shown in supplemental Fig. S1 (.png file). This includes 596 reactions and 250 N-linked glycans.