Fig. 3.

Annotated examples of MS/MS spectra with different type of glycopeptide assignments or under different fragmentation modes. A, HCD mode fragmentation of fetuin glycopeptide; B, CID mode example of core-fucosylated N-linked glycopeptide from fetuin; C, ETD mode N-glycopeptide of fetuin; D, Glycopeptide from asialofetuin; E, High mannose structure from RNase B; F, sialyated T-antigen glycopeptide of fetuin. Theoretical and experimental MS¹ m/z values are provided in figure. Trypsin was used for digestion in all panels, except panels B and E which used Glu-C.