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. 2017 Nov 1;11:609. doi: 10.3389/fnins.2017.00609

Figure 2.

Figure 2

Altered dendritic arbors and increased spine density of SOD1 XII MNs commencing from P8-15 compared to WT controls. Images show mosaics of XII MNs from P8-15, P28-35, P65-75, and P120 WT (A,C,E,G) and SOD1 (B,D,F,H) mice brainstem. Superimposed in purple are dendritic tracings from the XII MN in order to illustrate more clearly the dendritic arbor size differences. High-magnification images of dendrites from P8-15, P28-35, P65-75, and P120 WT (I,K,M,O) and SOD1 (J,L,N,P) XII MNs are also shown, with examples of some of the dendritic spines identified using red arrows. Note that this is representative of the spiny structures and not an exhaustive identification of all quantified spines on these images. (Q) shows a scatterplot quantifying significantly increased total dendritic length (μm) of SOD1 XII MNs (gray squared) at P8-15 and 28-35 and significant decreases at P120 compared to WT controls (white circles). (R) shows a scatterplot quantifying significantly increased mean tree dendritic length (μm) of SOD1 XII MNs (gray squared) at P8-15 and 28-35 and significant decreases at P120 compared to WT controls (white circles). (S) shows a scatterplot quantifying significantly increased dendritic spine density per 100 μm of SOD1 XII MNs (gray squared) compared to WT controls (white circles) at P8-15, P28-35, P65-75, and P120. All data mean ± SEM, with two-way ANOVAs followed by Bonferroni post-tests, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. n = 7, 5, 5, and 8 for WT P8-15, P28-35, P65-75, and P120 respectively. n = 6, 5, 5, and 12 for SOD1 P8-15, P28-35, P65-75, and P120 respectively. Scale bar: (A–H), 100 μm. Dendritic frame width (I–P), 15 μm.