Table 1.
Morphometric dendritic and dendritic spine parameters of MSNs within the striatum.
| Region | P8-15 (n) Neonatal | P28-35 (n) Pre-symptomatic | P65-75 (n) Symptom Onset | P120 (n) Mid-disease |
|---|---|---|---|---|
| Soma volume (μm3) | WT: 5932 ± 1122 (7) SOD1: 7065 ± 1937 (5) Not significant |
WT: 10170 ± 1904 (5) SOD1: 5867 ± 698 (5) Not significant |
WT: 7520 ± 1344 (5) SOD1: 7907 ± 1357 (5) Not significant |
WT: 6039 ± 1218 (8) SOD1: 7763 ± 623 (12) Not significant |
| Total arbor length (μm) | WT: 904 ± 125 (7) SOD1: 1119 ± 195 (5) Not significant |
WT: 1701 ± 298 (5) SOD1: 2309 ± 218 (5) Not significant |
WT: 1068 ± 168 (5) SOD1: 1444 ± 351 (5) Not significant |
WT: 1241 ± 121 (8) SOD1: 944 ± 139 (12) Not significant |
| Mean tree length (μm) | WT: 236 ± 30 (7) SOD1: 378 ± 79 (5) Not significant |
WT: 379 ± 65 (5) SOD1: 485 ± 65 (5) Not significant |
WT: 322 ± 47 (5) SOD1: 453 ± 94 (5) Not significant |
WT: 335 ± 45 (8) SOD1: 249 ± 41 (12) Not significant |
| Dendritic reach (μm) | WT: 154 ± 19 (7) SOD1: 255 ± 51 (5) Not significant |
WT: 296 ± 36 (5) SOD1: 321 ± 23 (5) Not significant |
WT: 171 ± 20 (5) SOD1: 190 ± 24 (5) Not significant |
WT: 227 ± 25 (8) SOD1: 157 ± 16 (12) Not significant |
| Dendritic ramifications | WT: 3.6 ± 0.4 (7) SOD1: 3.3 ± 0.3 (5) Not significant |
WT: 3.8 ± 0.3 (5) SOD1: 4.6 ± 0.3 (5) Not significant |
WT: 3.8 ± 0.4 (5) SOD1: 4.2 ± 0.5 (5) Not significant |
WT: 4.5 ± 0.4 (8) SOD1: 4.6 ± 0.3 (12) Not significant |
| Dendritic spine density per 100 μm | WT: 40 ± 3.6 (7) SOD1: 33.8 ± 3.8 (5) Adj. P > 0.9999 |
WT: 57 ± 6.5 (5) SOD1: 43.8 ± 4.5 (5) Adj. P = 0.2936 |
WT: 58.4 ± 6.9 (5) SOD1: 34.8 ± 4.1 (5) Adj. P = 0.0082** |
WT: 42.4 ± 5.4 (8) SOD1: 28.6 ± 2.3 (12) Adj. P = 0.0428* |
All data presented as mean ± SEM. All analyses were unpaired two-way ANOVAs for age and genotype, comparing soma volume (Age: P = 0.6285; Genotype: P = 0.7725), total dendritic arbor length (Age: P < 0.0001****; Genotype: P = 0.1201), mean dendritic tree length (Age: P = 0.0575; Genotype: P = 0.0824), dendritic reach (Age: P = 0.0002***; Genotype: P = 0.4472), dendritic ramifications (Age: P = 0.0379*; Genotype: P = 0.6085), and dendritic spine density per 100 μm (Age: P = 0.0041**; Genotype: P < 0.0001****). Bonferroni post-tests were used to compare the effect of genotype at each age group, with the adjusted P-value (adj. P) reported in the table for parameters that had significant genotype effects. Each animal used (n) contains mean values from multiple individual neurons, specifically P8-15 (WT: 11 neurons; SOD1: 12 neurons), P28-35 (WT: 8 neurons; SOD1: 9 neurons), P65-75 (WT: 9 neurons; SOD1: 10 neurons), and P120 (WT: 12 neurons; SOD1: 16 neurons).