Table 4.
Morphometric dendritic and dendritic spine parameters of MNs in the lumbar spinal cord.
| Region | P8-15 (n) Neonatal | P28-35 (n) Pre-symptomatic | P65-75 (n) Symptom onset | P120 (n) Mid-disease |
|---|---|---|---|---|
| Soma volume (μm3) | WT: 17937 ±3476 (7) SOD1: 16895 ± 1966 (5) Adj. P > 0.9999 |
WT: 46512 ± 3182 (5) SOD1: 36391 ± 2769 (5) Adj. P = 0.1530 |
WT: 38309 ± 5299 (5) SOD1: 43454 ± 3571 (5) Adj. P = 0.9999 |
WT: 31198 ± 2331 (8) SOD1: 13659 ± 1816 (12) Adj. P < 0.0001**** |
| Total arbor length (μm) | WT: 2195 ± 205 (7) SOD1: 1873 ± 149 (5) Adj. P > 0.9999 |
WT: 4945 ± 836 (5) SOD1: 2828 ± 314 (5) Adj. P = 0.0226* |
WT: 6604 ± 1090 (5) SOD1: 3231 ± 427 (5) Adj. P = 0.0001*** |
WT: 4971 ± 299 (8) SOD1: 1778 ± 240 (12) Adj. P < 0.0001**** |
| Mean tree length (μm) | WT: 560 ± 41 (7) SOD1: 490 ± 36 (5) Adj. P > 0.9999 |
WT: 1315 ± 118 (5) SOD1: 826 ± 151 (5) Adj. P = 0.0023** |
WT: 1353 ± 179 (5) SOD1: 758 ± 113 (5) Adj. P = 0.0009*** |
WT: 1073 ± 102 (8) SOD1: 380 ± 24 (12) Adj. P < 0.0001**** |
| Dendritic reach (μm) | WT: 351 ± 40 (7) SOD1: 330 ± 32 (5) Adj. P > 0.9999 |
WT: 595 ± 58 (5) SOD1: 413 ± 23 (5) Adj. P = 0.0334* |
WT: 756 ± 83 (5) SOD1: 517 ± 55 (5) Adj. P = 0.0032** |
WT: 562 ± 32 (8) SOD1: 302 ± 21 (12) Adj. P = 0.0001**** |
| Dendritic ramifications | WT: 4.7 ± 0.4 (7) SOD1: 4.3 ± 0.2 (5) Adj. P > 0.9999 |
WT: 5.2 ± 0.6 (5) SOD1: 4.6 ± 0.1 (5) Adj. P > 0.9999 |
WT: 7.2 ± 0.6 (5) SOD1: 5.0 ± 0.3 (5) Adj. P = 0.0176* |
WT: 7.4 ± 0.7 (8) SOD1: 4.3 ± 0.2 (12) Adj. P = 0.0001**** |
| Dendritic spine density per 100 μm | WT: 11.0 ± 1.1 (7) SOD1: 16.3 ± 1.5 (5) Adj. P = 0.0374* |
WT: 10.8 ± 0.9 (5) SOD1: 7.6 ± 1.8 (5) Adj. P = 0.4785 |
WT: 11.5 ± 1.6 (5) SOD1: 7.4 ± 1 (5) Adj. P = 0.2205 |
WT: 12.4 ± 1.5 (8) SOD1: 6.8 ± 0.9 (12) Adj. P = 0.0021** |
All data are mean ± SEM. All analyses were unpaired two-way ANOVAs for age and genotype, comparing soma volume (Age: P < 0.0001****; Genotype: P = 0.0097**), total dendritic arbor length (Age: P < 0.0001****; Genotype: P < 0.0001****), mean dendritic tree length (Age: P < 0.0001****; Genotype: P < 0.0001****), dendritic reach (Age: P < 0.0001****; Genotype: P < 0.0001****), dendritic ramifications (Age: P = 0.0036**; Genotype: P = 0.0001****), and dendritic spine density per 100 μm (Age: P = 0.0051**; Genotype: P < 0.0481*). Bonferroni post-tests were used to compare the effect of genotype at each age group, with the adjusted P-value (adj. P) reported in the table for parameters that had significant genotype effects. Each animal used (n) contains mean values from a minimum of 2 individual neurons, specifically P8-15 (WT: 25 neurons; SOD1: 13 neurons), P28-35 (WT: 12 neurons; SOD1: 18 neurons), P65-75 (WT: 13 neurons; SOD1: 19 neurons), and P120 (WT: 30 neurons; SOD1: 39 neurons).