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. 2017 Nov 1;11:609. doi: 10.3389/fnins.2017.00609

Table 5.

Morphometric dendritic and dendritic spine parameters of IV MNs within the brainstem.

Region P8-15 (n) Neonatal P28-35 (n) Pre-symptomatic P65-75 (n) Symptom onset P120 (n) Mid-disease
Soma volume (μm3) WT: 21787 ± 2174 (7)
SOD1: 18415 ± 2391 (5)
Not significant		 WT: 24493 ± 2000 (5)
SOD1: 21604 ± 2394 (5)
Not significant		 WT: 29634 ± 3835 (5)
SOD1: 24497 ± 4541 (5)
Not significant		 WT: 26868 ± 1150 (8)
SOD1: 25378 ± 2525 (12)
Not significant
Total arbor length (μm) WT: 1370 ± 156 (7)
SOD1: 1119 ± 129 (5)
Not significant		 WT: 1495 ± 181 (5)
SOD1: 1563 ± 130 (5)
Not significant		 WT: 2053 ± 230 (5)
SOD1: 1714 ± 83 (5)
Not significant		 WT: 1728 ± 116 (8)
SOD1: 1686 ± 150 (12)
Not significant
Mean tree length (μm) WT: 371 ± 44 (7)
SOD1: 275 ± 17 (5)
Not significant		 WT: 342 ± 53 (5)
SOD1: 392 ± 62 (5)
Not significant		 WT: 529 ± 55 (5)
SOD1: 446 ± 50 (5)
Not significant		 WT: 370 ± 24 (8)
SOD1: 472 ± 45 (12)
Not significant
Dendritic reach (μm) WT: 157 ± 12 (7)
SOD1: 133 ± 9 (5)
Not significant		 WT: 170 ± 7 (5)
SOD1: 194 ± 8 (5)
Not significant		 WT: 270 ± 14 (5)
SOD1: 232 ± 7 (5)
Not significant		 WT: 215 ± 10 (8)
SOD1: 202 ± 13 (12)
Not significant
Dendritic ramifications WT: 3.7 ± 0.2 (7)
SOD1: 3.8 ± 0.3 (5)
Not significant		 WT: 3.4 ± 0.5 (5)
SOD1: 4.5 ± 0.5 (5)
Not significant		 WT: 3.2 ± 0.3 (5)
SOD1: 3.2 ± 0.4 (5)
Not significant		 WT: 3.6 ± 0.1 (8)
SOD1: 3.9 ± 0.3 (12)
Not significant
Dendritic spine density per 100 μm WT: 10.6 ± 3.6 (7)
SOD1: 13.4 ± 5 (5)
Not significant		 WT: 10.6 ± 1.5 (5)
SOD1: 15 ± 3.2 (5)
Not significant		 WT: 13 ± 1.5 (5)
SOD1: 13.8 ± 1.5 (5)
Not significant		 WT: 8.6 ± 1.9 (8)
SOD1: 9.4 ± 1.5 (12)
Not significant

All data presented as mean ± SEM. All analyses were unpaired two-way ANOVAs for age and, comparing soma volume (Age: P = 0.0732; Genotype: P = 0.1188), total dendritic arbor length (Age: P = 0.0043**; Genotype: P = 0.3305), mean dendritic tree length (Age: P = 0.0183*; Genotype: P = 0.8501), dendritic reach (Age: P < 0.0001****; Genotype: P = 0.1711), dendritic ramifications (Age: P = 0.2077; Genotype: P = 0.1418), and dendritic spine density per 100 μm (Age: P = 0.2580; Genotype: P = 0.2489). Bonferroni post-tests were used to compare the effect of genotype at each age group, with the adjusted P-value (adj. P) reported in the table for parameters that had significant genotype effects. Each animal used (n) contains mean values from multiple neurons, specifically P8-15 (WT: 9 neurons; SOD1: 6 neurons), P28-35 (WT: 6 neurons; SOD1: 8 neurons), P65-75 (WT: 6 neurons; SOD1: 6 neurons), and P120 (WT: 11 neurons; SOD1: 15 neurons).