Table 3.
Steady-state kinetics of hpol κ incorporation of nucleotides
| Primer/template | dNTP | Km | kcat | kcat/Km | -Fold changea | fb |
|---|---|---|---|---|---|---|
| μm | min−1 | μm−1 min−1 | -fold | |||
| RNA/DNA (dG) | dCTP | 330 ± 42 | 4.5 ± 0.2 | 0.014 ± 0.002 | 260 | |
| DNA/RNA (rG) | dCTP | 726 ± 145 | 1.1 ± 0.1 | 0.0015 ± 0.0003 | 2,400 | |
| DNA/DNA (dG) | dCTP | 0.47 ± 0.04 | 1.7 ± 0.1 | 3.6 ± 0.4 | ||
| RNA/DNA (8-oxodG) | dCTP | 618 ± 86 | 1.3 ± 0.1 | 0.0021 ± 0.0003 | 1 | |
| RNA/ DNA (8-oxodG) | dATP | 497 ± 81 | 1.1 ± 0.1 | 0.0022 ± 0.0004 | 1.05 | |
| DNA/DNA (8-oxodG) | dCTP | 8.9 ± 0.9 | 2.1 ± 0.1 | 0.24 ± 0.03 | 1 | |
| DNA/DNA (8-oxodG) | dATP | 0.75 ± 0.08 | 1.7 ± 0.1 | 2.3 ± 0.3 | 9.6 |
a -Fold change = (kcat/Km)hybrid/(kcat/Km)DNA/DNA.
b Misinsertion frequency: f = (kcat/Km)incorrect/(kcat/Km)correct.