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Endocytosis inhibition results in mTORC1 inactivation. A, 293T cells were transfected with pcDNA3.1 vector (mock) or pcDNA3.1/dynamin 2 K44A. Cells were harvested at 24 h after transfection. B, quantification of the immunoblot data in A. Each bar indicates mean ± S.D. of three independent experiments. *, p < 0.05. C, 293T cells were treated with 0.1% DMSO (vehicle), 80 μm Dynasore, or 30 μm PitStop 2 for the indicated time.
