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. 2017 Sep 11;292(44):18129–18144. doi: 10.1074/jbc.M117.784546

Table 2.

Relative levels of proteins involved in the regulation of rRNA processing in cells treated with the SIRT1 inhibitor EX-527 relative to control

The reported values correspond to the ratio of intensities for each protein between treated and control cells (EX-527/CTRL) for the three cell lines under study.

Accession number Gene Protein names Unique peptides Molecular mass (kDa) Q-value Score EX-527/CTRL
HaCaT CaLo SiHa
47S pre-rRNA processing to yield 45S pre-rRNA
    Q9H8H0 NOL11 Nucleolar protein 11 2 81.123 0 96.59 2.11 1.19 2.74
    Q9Y5J1 UTP18 U3 small nucleolar RNA-associated protein 18 homolog 9 62.003 0 75.95 0.91 1.14 5.03

45S pre-rRNA processing to yield 30S and 32S pre-RNAs
    Q9NW13 RBM28 RNA-binding protein 28 15 85.737 0 212.16 1.16 0.95 1.25
    Q01780 EXOSC10 Exosome component 10 21 100.83 0 180.36 1.85 1.36 1.41
    Q9UGY1 NOL12 Nucleolar protein 12 14 24.663 0 40.817 1.52 1.28 1.47
    P56182 RRP1 Ribosomal RNA processing protein 1 homolog A 22 52.839 0 223.67 1.26 1.16 2.17
    Q14684 RRP1B Ribosomal RNA processing protein 1 homolog B 18 82.175 0 157.89 1.51 1.57 2.08
    Q14137 BOP1 Ribosome biogenesis protein BOP1 22 83.629 0 116.56 1.68 1.47 1.70
    Q9H0D6 XRN2 5′–3′ Exoribonuclease 2 28 108.58 0 288.2 1.31 0.64 1.11
    Q9Y2R4 DDX52 Probable ATP-dependent RNA helicase DDX52 11 67.497 0 30.176 1.85 1.55 2.10

30S pre-rRNA processing to yield 21S pre-rRNA
    P42677 RPS27 40S ribosomal protein S27 3 9.461 0 130.44 0.66 1.27 1.03
    P15880 RPS2 40S ribosomal protein S2 8 31.324 0 157.79 9.53 1.01 1.25
    P62847 RPS24 40S ribosomal protein S24 17 15.423 0 126.66 0.69 1.34 1.16
    Q9H8H0 NOL11 Nucleolar protein 11 2 81.123 0 96.59 2.11 1.19 2.74
    P62081 RPS7 40S ribosomal protein S7 11 21.312 0 117.54 1.51 1.56 4.02
    Q9Y324 FCF1 rRNA-processing protein FCF1 homolog 13 23.369 0 35.869 1.86 3.88 5.03
    P62249 RPS16 40S ribosomal protein S16 14 16.445 0 323.31 1.18 1.51 1.56
    P62277 RPS13 40S ribosomal protein S13 10 17.222 0 46.946 3.30 2.36 3.09
    P61247 RPS3A 40S ribosomal protein S3a 11 23.747 0 323.31 0.94 1.56 1.14
    P46782 RPS5 40S ribosomal protein S5 8 25.333 0 118.21 1.77 2.45 2.34
    P62857 RPS28 40S ribosomal protein S28 5 7.8409 0 193.73 0.77 0.84 1.08
    P62244 RPS15A 40S ribosomal protein S15a 8 14.839 0 309.07 1.45 1.58 0.70
    P62241 RPS8 40S ribosomal protein S8 19 24.205 0 323.31 0.67 1.35 1.03
    P62280 RPS11 40S ribosomal protein S11 11 18.431 0 121.11 2.67 0.88 2.12
    Q8IY81 FTSJ3 Pre-rRNA processing protein FTSJ3 30 96.557 0 196.05 1.80 1.19 1.57
    P62753 RPS6 40S ribosomal protein S6 16 28.68 0 323.31 1.11 0.76 1.02

21S pre-rRNA processing to yield 18S pre-rRNA
    Q9NQT4 EXOSC5 Exosome complex component RRP46 5 25.249 0 13.818 2.27 2.89 1.07
    P42285 SKIV2L2 Superkiller viralicidic activity 2-like 2 19 117.8 0 230.06 0.96 1.06 1.15
    Q01780 EXOSC10 Exosome component 10 21 100.83 0 180.36 1.85 1.36 1.41

18S pre-rRNA processing in pre-40S particles
    O43709 WBSCR22 Probable 18S rRNA (guanine-N7)-methyltransferase 5 31.88 0 30.62 2.20 0.99 2.36

32S pre-rRNA processing to yield 12S pre-rRNA and 28S rRNA
    Q9BVP2 GNL3 Guanine nucleotide-binding protein-like 3 16 61.992 0 165 1.83 0.84 1.63
    Q9NVN8 GNL3L Guanine nucleotide-binding protein-like 3-like protein 10 65.572 0 20.152 4.54 0.76 12.52
    Q99848 EBNA1BP2 Probable rRNA-processing protein EBP2 23 40.684 0 279.31 1.04 1.08 1.46
    Q9NR30 DDX21 Nucleolar RNA helicase 2 17 87.343 0 323.31 1.84 0.68 3.49
    O00541 PES1 Pescadillo homolog 31 66.077 0 284.25 2.68 0.91 2.50
    Q9Y4W2 LAS1L Ribosomal biogenesis protein LAS1L 18 81.242 0 57.623 2.03 1.02 1.28
    Q5SY16 NOL9 Polynucleotide 5-hydroxyl kinase NOL9 12 79.322 0 110.49 2.11 1.47 0.75

12S pre-rRNA processing to yield 5.8S rRNA
    P42285 SKIV2L2 Superkiller viralicidic activity 2-like 2 19 117.8 0 230.06 0.96 1.06 1.15
    Q9Y2L1 DIS3 Exosome complex exonuclease RRP44 20 109 0 115.25 0.63 0.73 0.51
    Q99547 MPHOSPH6 M-phase phosphoprotein 6 5 19.024 0.0004 3.9044 1.29 2.56 1.55
    Q01780 EXOSC10 Exosome component 10 21 100.83 0 180.36 1.85 1.36 1.41