Table 2.
Relative levels of proteins involved in the regulation of rRNA processing in cells treated with the SIRT1 inhibitor EX-527 relative to control
The reported values correspond to the ratio of intensities for each protein between treated and control cells (EX-527/CTRL) for the three cell lines under study.
| Accession number | Gene | Protein names | Unique peptides | Molecular mass (kDa) | Q-value | Score | EX-527/CTRL |
||
|---|---|---|---|---|---|---|---|---|---|
| HaCaT | CaLo | SiHa | |||||||
| 47S pre-rRNA processing to yield 45S pre-rRNA | |||||||||
| Q9H8H0 | NOL11 | Nucleolar protein 11 | 2 | 81.123 | 0 | 96.59 | 2.11 | 1.19 | 2.74 |
| Q9Y5J1 | UTP18 | U3 small nucleolar RNA-associated protein 18 homolog | 9 | 62.003 | 0 | 75.95 | 0.91 | 1.14 | 5.03 |
| 45S pre-rRNA processing to yield 30S and 32S pre-RNAs | |||||||||
| Q9NW13 | RBM28 | RNA-binding protein 28 | 15 | 85.737 | 0 | 212.16 | 1.16 | 0.95 | 1.25 |
| Q01780 | EXOSC10 | Exosome component 10 | 21 | 100.83 | 0 | 180.36 | 1.85 | 1.36 | 1.41 |
| Q9UGY1 | NOL12 | Nucleolar protein 12 | 14 | 24.663 | 0 | 40.817 | 1.52 | 1.28 | 1.47 |
| P56182 | RRP1 | Ribosomal RNA processing protein 1 homolog A | 22 | 52.839 | 0 | 223.67 | 1.26 | 1.16 | 2.17 |
| Q14684 | RRP1B | Ribosomal RNA processing protein 1 homolog B | 18 | 82.175 | 0 | 157.89 | 1.51 | 1.57 | 2.08 |
| Q14137 | BOP1 | Ribosome biogenesis protein BOP1 | 22 | 83.629 | 0 | 116.56 | 1.68 | 1.47 | 1.70 |
| Q9H0D6 | XRN2 | 5′–3′ Exoribonuclease 2 | 28 | 108.58 | 0 | 288.2 | 1.31 | 0.64 | 1.11 |
| Q9Y2R4 | DDX52 | Probable ATP-dependent RNA helicase DDX52 | 11 | 67.497 | 0 | 30.176 | 1.85 | 1.55 | 2.10 |
| 30S pre-rRNA processing to yield 21S pre-rRNA | |||||||||
| P42677 | RPS27 | 40S ribosomal protein S27 | 3 | 9.461 | 0 | 130.44 | 0.66 | 1.27 | 1.03 |
| P15880 | RPS2 | 40S ribosomal protein S2 | 8 | 31.324 | 0 | 157.79 | 9.53 | 1.01 | 1.25 |
| P62847 | RPS24 | 40S ribosomal protein S24 | 17 | 15.423 | 0 | 126.66 | 0.69 | 1.34 | 1.16 |
| Q9H8H0 | NOL11 | Nucleolar protein 11 | 2 | 81.123 | 0 | 96.59 | 2.11 | 1.19 | 2.74 |
| P62081 | RPS7 | 40S ribosomal protein S7 | 11 | 21.312 | 0 | 117.54 | 1.51 | 1.56 | 4.02 |
| Q9Y324 | FCF1 | rRNA-processing protein FCF1 homolog | 13 | 23.369 | 0 | 35.869 | 1.86 | 3.88 | 5.03 |
| P62249 | RPS16 | 40S ribosomal protein S16 | 14 | 16.445 | 0 | 323.31 | 1.18 | 1.51 | 1.56 |
| P62277 | RPS13 | 40S ribosomal protein S13 | 10 | 17.222 | 0 | 46.946 | 3.30 | 2.36 | 3.09 |
| P61247 | RPS3A | 40S ribosomal protein S3a | 11 | 23.747 | 0 | 323.31 | 0.94 | 1.56 | 1.14 |
| P46782 | RPS5 | 40S ribosomal protein S5 | 8 | 25.333 | 0 | 118.21 | 1.77 | 2.45 | 2.34 |
| P62857 | RPS28 | 40S ribosomal protein S28 | 5 | 7.8409 | 0 | 193.73 | 0.77 | 0.84 | 1.08 |
| P62244 | RPS15A | 40S ribosomal protein S15a | 8 | 14.839 | 0 | 309.07 | 1.45 | 1.58 | 0.70 |
| P62241 | RPS8 | 40S ribosomal protein S8 | 19 | 24.205 | 0 | 323.31 | 0.67 | 1.35 | 1.03 |
| P62280 | RPS11 | 40S ribosomal protein S11 | 11 | 18.431 | 0 | 121.11 | 2.67 | 0.88 | 2.12 |
| Q8IY81 | FTSJ3 | Pre-rRNA processing protein FTSJ3 | 30 | 96.557 | 0 | 196.05 | 1.80 | 1.19 | 1.57 |
| P62753 | RPS6 | 40S ribosomal protein S6 | 16 | 28.68 | 0 | 323.31 | 1.11 | 0.76 | 1.02 |
| 21S pre-rRNA processing to yield 18S pre-rRNA | |||||||||
| Q9NQT4 | EXOSC5 | Exosome complex component RRP46 | 5 | 25.249 | 0 | 13.818 | 2.27 | 2.89 | 1.07 |
| P42285 | SKIV2L2 | Superkiller viralicidic activity 2-like 2 | 19 | 117.8 | 0 | 230.06 | 0.96 | 1.06 | 1.15 |
| Q01780 | EXOSC10 | Exosome component 10 | 21 | 100.83 | 0 | 180.36 | 1.85 | 1.36 | 1.41 |
| 18S pre-rRNA processing in pre-40S particles | |||||||||
| O43709 | WBSCR22 | Probable 18S rRNA (guanine-N7)-methyltransferase | 5 | 31.88 | 0 | 30.62 | 2.20 | 0.99 | 2.36 |
| 32S pre-rRNA processing to yield 12S pre-rRNA and 28S rRNA | |||||||||
| Q9BVP2 | GNL3 | Guanine nucleotide-binding protein-like 3 | 16 | 61.992 | 0 | 165 | 1.83 | 0.84 | 1.63 |
| Q9NVN8 | GNL3L | Guanine nucleotide-binding protein-like 3-like protein | 10 | 65.572 | 0 | 20.152 | 4.54 | 0.76 | 12.52 |
| Q99848 | EBNA1BP2 | Probable rRNA-processing protein EBP2 | 23 | 40.684 | 0 | 279.31 | 1.04 | 1.08 | 1.46 |
| Q9NR30 | DDX21 | Nucleolar RNA helicase 2 | 17 | 87.343 | 0 | 323.31 | 1.84 | 0.68 | 3.49 |
| O00541 | PES1 | Pescadillo homolog | 31 | 66.077 | 0 | 284.25 | 2.68 | 0.91 | 2.50 |
| Q9Y4W2 | LAS1L | Ribosomal biogenesis protein LAS1L | 18 | 81.242 | 0 | 57.623 | 2.03 | 1.02 | 1.28 |
| Q5SY16 | NOL9 | Polynucleotide 5-hydroxyl kinase NOL9 | 12 | 79.322 | 0 | 110.49 | 2.11 | 1.47 | 0.75 |
| 12S pre-rRNA processing to yield 5.8S rRNA | |||||||||
| P42285 | SKIV2L2 | Superkiller viralicidic activity 2-like 2 | 19 | 117.8 | 0 | 230.06 | 0.96 | 1.06 | 1.15 |
| Q9Y2L1 | DIS3 | Exosome complex exonuclease RRP44 | 20 | 109 | 0 | 115.25 | 0.63 | 0.73 | 0.51 |
| Q99547 | MPHOSPH6 | M-phase phosphoprotein 6 | 5 | 19.024 | 0.0004 | 3.9044 | 1.29 | 2.56 | 1.55 |
| Q01780 | EXOSC10 | Exosome component 10 | 21 | 100.83 | 0 | 180.36 | 1.85 | 1.36 | 1.41 |