Figure 3.
The kpol and Kd values of correct nucleotide incorporation by POLRMT. A, single-turnover kinetics of UTP incorporation into 100 nm dA-ES by 200 nm POLRMT. The single-turnover kinetics were measured at increasing concentrations of UTP at 25 °C in a rapid chemical quench-flow instrument, and the data were fit to a single exponential equation to obtain the rate constants. FAM, 6-carboxyfluorescein. B, the rate constants from A are plotted against [UTP], and the dependence was fit to a hyperbola to obtain the indicated kpol and Kd of correct UTP incorporation by POLRMT. The errors are standard errors of fitting. The experiment was carried out twice and representative data are shown. Error bars represent S.E.