Figure 3.

Proteins with a broad range of functions are phosphorylated in a GSK-3–dependent manner in mouse ESCs. A, gene ontology analysis of proteins with significantly reduced phosphorylation in Gsk3 DKO cells using DAVID. B, STRING analysis of proteins enriched in the GO analysis generates three networks of proteins with related functions. Darker hue indicates greater reduction in phosphorylation in DKO cells. The red asterisk indicates phosphorylation sites with GSK-3 consensus motif. Phosphorylation of proteins in this analysis was reduced >1.5-fold with p value ≤ 0.05.