Figure 7.
Effects of AGAP and W38G on hNav1.7. The Na+ currents were obtained by plotting current peak amplitudes with a function of test potentials ranging from −80 to +80 mV for 50 ms from the holding potential of −80 mV in increments of 10 mV. The current traces were evoked by −20 mV for 50 ms from a holding potential of −80 mV in the absence and presence of 100 nm AGAP and W38G. Averaged current traces were obtained from hNav1.7 (A) cells in each group. The current-voltage relationship of hNav1.7 (B) was evoked by 100 nm AGAP and W38G. Peak currents were converted into conductance, and normalized conductance of hNav1.7 (C) was plotted against the voltages of conditioning pulses. The currents were elicited with a test pulse at −20 mV for 20 ms following 30-ms prepulses ranging from −100 to 0 mV at 5-mV steps. Peak currents were normalized, and the inactivation curves of hNav1.7 (D) were plotted against the command potentials. Each data point represents mean ± S.E. (error bars) (n = 6).