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. 2017 Sep 15;292(44):18344–18353. doi: 10.1074/jbc.M117.813444

Figure 1.

Figure 1.

Purification of recombinant His-tagged ApoL1. A, protein-stained gel of fractions from purification. Lane 1, peak fraction from nickel column elution. Lane 2, molecular mass standards (from the top: 100, 75, 63, 48, 35, and 28 kDa). Lanes 3–23, fractions 29 through 50 from the S200 column (every other lane is labeled with fraction number). ApoL1 peaks in fractions 38–39, running just below the 48-kDa marker. B, protein concentration of S200 fractions. C, chloride permease activity of S200 fractions 32–44. Each data point represents the mean, with error bars indicating S.E.; n = 2 for each. D, potassium permease activity of S200 fractions 32–44. Data are as in C; n = 3 for each data point.