Figure 1.

Res-006 exhibits a higher cytotoxic activity than Res and Res-005 in hepatocellular carcinoma cells. (A) Chemical structures of Res and the two derivatives. (B), (D), and (F) THLE-2, Huh-7, and HepG2 cells were treated with Res, Res-005, or Res-006 (0–300 μmol/L) for 24 h, and cell viability was analyzed by the CCK-8 assay. The data are expressed as the mean±SEM of three independent experiments, ^***P<0.001; 0 μmol/L vs each concentration. (C), (E), and (G) Dose response curves and EC₅₀ values of Res-006 for THLE-2, Huh-7, and HepG2 cells. The data are expressed as the mean±SEM of three independent experiments. (H) THLE-2, Huh-7, and HepG2 cells were treated with Res-006 (65 μmol/L) for 24 h, and cell viability was analyzed by the CCK-8 assay. The data are expressed as the mean±SEM of three independent experiments, ^***P<0.001; Mock-treated cells vs Res-006-treated cells. (I) THLE-2, Huh-7, and HepG2 cells were treated with Res-006 (65 μmol/L) for the indicated times, and total cellular extracts were prepared and subjected to Western blot analysis.