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. 2017 Aug 10;38(11):1486–1500. doi: 10.1038/aps.2017.106

Figure 6.

Figure 6

Res-006 is a strong ER stress inducer. (A and B) HepG2 cells were treated with Res-006 (65 μmol/L), and total cellular extracts and total RNAs were prepared and subjected to Western blot and semi-quantitative PCR analysis. (C) HepG2 cells were transfected with pCMVFlag3x-ATF6α for 48 h and then treated with DTT (5 mmol/L) or Res-006 (65 μmol/L) for the indicated times. Total cellular extracts were prepared and subjected to Western blot analysis. (D) HepG2 cells were treated with Res-006 (65 μmol/L) for the indicated times. Total RNA was prepared and subjected to quantitative real-time (qRT)-PCR analysis. The data are expressed as the mean±SEM of four independent experiments, *P<0.05 and ***P<0.001; zero time vs each time.