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. 2017 Nov 4;19(4):537–544. doi: 10.22074/cellj.2018.4130

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Fig.6 — Cerebrospinal fluid (CSF) induced neuronal differentiation in the bone marrow mesenchymal stem cells (BM-MSCs). A. β-III tubulin expression in BMMSCs cultured with 10 ng/ml basic fibroblast growth factor (b-FGF) (×400), B. 10% E19 CSF (×400), C. 10% E20 CSF (×400), D. 10% P1 CSF (×400), E. MAP-2 expression in BM-MSCs cultured with 10 ng/ml b-FGF (×400), F. 10% E19 CSF (×400), G. 10% E20 CSF (×400), H. 10% P1 CSF (×400), and I. Control (×200) cultures without added CSF cells that show no stain when primary antibody was omitted. Red arrows indicate positive expression of β-III tubulin and MAP-2 in neuronlike cells and neurites. The highest expression level for these markers was detected in E19.