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Gradient program for HPLC separation and the quantification of amino acids and ammonium.
| Time (min) | Buffer A (%) | Buffer B (%) | Water (%) |
|---|---|---|---|
| 0 | 100 | 0 | 0 |
| 1 | 100 | 0 | 0 |
| 1.5 | 99 | 1 | 0 |
| 16.5 | 97 | 3 | 0 |
| 25.5 | 94 | 6 | 0 |
| 35.5 | 86 | 14 | 0 |
| 51.5 | 86 | 14 | 0 |
| 52 | 0 | 60 | 40 |
| 55 | 0 | 60 | 40 |
| 55.5 | 100 | 0 | 0 |
| 66.5 | 100 | 0 | 0 |
