Figure 3.

Spontaneous GABA_A receptor PSCs (sGPSCs) recorded from GnRH neurons do not change in the different mouse models of estrogen negative and positive feedback. A-D, Representative examples of sGPSC current recordings in the presence of AP5 (50 μM) and CNQX (10 μM) taken from diestrous, OVX, OVX + E, and OVX + E+E mice. Underneath each trace, an enlarged time scale of one GPSC (*) is shown. E, F, Cumulative plots of the average inter-sGPSC interval (G) and amplitude (H) in the different experimental groups (for clarity only positive SEM is shown). N = 7 for each group. Cell numbers are shown in parenthesis. Numbers of PSCs analyzed is given in Table 1. No statistically significant differences were detected for any parameter between groups.