Figure 5.

Miniature excitatory glutamate receptor PSCs (mEPSCs) recorded from GnRH neurons do not change in the different mouse models of estrogen negative and positive feedback. A-F, Representative examples of mEPSCs current recordings in the presence of GABAzine (5 μM) and TTX (0.5 μM) taken from diestrous, OVX, OVX + E, and OVX + E+E mice. Underneath each trace an enlarged time scale of one mEPSC (*) is shown. C, F, mEPSCs are abolished by AP5 (50 μM) + CNQX (10 μM). G, H, Cumulative plots of the average inter-mEPSC interval (G) and amplitude (H) in the different experimental groups (for clarity only positive SEM is shown). N = 5 for each group. Cell numbers are shown in parenthesis. Numbers of PSCs analyzed is given in Table 2. No statistically significant differences were detected for any parameter between groups.