The frequency of excitatory glutamate miniature (mEPSCs) and spontaneous PSCs (sEPSCs) recorded from GnRH neurons do not change in the different mouse models of estrogen negative and positive feedback. A, D, Histograms showing the percentage of GnRH neurons with mEPSCs (A) and EPSCs (D) in each group. B, E, Scatter plots showing the individual and mean (±SEM) frequency of mEPSCs (B) or EPSCs (E) in all GnRH neurons of the four experimental groups. C, F, Histograms showing the mean frequency of mEPSCs (C) or sEPSCs (F) from only cells exhibiting mEPSCs (C) or sEPSCs (F) in each animal group. Numbers at the base show the cell number. The animal number is five for each group. No statistically significant differences were detected for any parameter between the different groups.