Table 1.
Linear mixed effects modeling table of coefficient, SE, and t value calculated from the linear mixed effect model for data from: TUNEL assay (Fig. 1), caspase-3 staining (Fig. 1), CUX1 and CTIP2 staining (Fig. 3), superficial/deep layer area ratio (Fig. 3), synapse counting (Fig. 4), fEPSP %epileptiform (Fig. 6), and fEPSP area (Fig. 6)
| Linear mixed model fit | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Fixed effect | Coefficient | SE | t value | Significant | Fixed effect | Coefficient | SE | t value | Significant |
| TUNEL | Caspase-3 | ||||||||
| FL Injury | 4.88E-02 | 1.29E-02 | 3.798 | * | FL Injury | 5.17E-03 | 7.52E-04 | 6.870 | * |
| GBP Treatment | −1.14E-02 | 1.31E-02 | −0.871 | GBP treatment | -7.84E-04 | 7.69E-04 | -1.019 | ||
| α2δ-1 KO | −1.09E-02 | 1.31E-02 | −0.831 | α2δ-1 KO | −6.09E-04 | 8.46E-04 | −0.720 | ||
| Interaction GBP/FL | −1.92E-02 | 1.48E-02 | −1.298 | Interaction GBP/FL | −2.18E-03 | 9.11E-04 | −2.394 | * | |
| Interaction KO/FL | −2.20E-02 | 1.48E-02 | −1.483 | Interaction KO/FL | −2.03E-03 | 9.28E-04 | −2.193 | * | |
| Interaction GBP/KO | 2.24E-02 | 1.48E-02 | 1.514 | Interaction GBP/KO | 1.61E-03 | 9.24E-04 | 1.747 | ||
| CUX1 PMZ | CTIP2 PMZ | ||||||||
| FL Injury | −3.53E-03 | 9.94E-04 | −3.550 | * | FL Injury | −3.40E-03 | 8.31E-04 | −4.096 | * |
| GBP treatment | 1.35E-04 | 9.14E-04 | 0.148 | GBP treatment | −2.35E-04 | 7.38E-04 | −0.318 | ||
| α2δ-1 KO | 1.42E-03 | 9.86E-04 | 1.438 | α2δ-1 KO | 7.33E-04 | 8.27E-04 | 0.887 | ||
| Interaction GBP/FL | 3.05E-03 | 1.13E-03 | 2.710 | * | Interaction GBP/FL | 1.09E-03 | 9.38E-04 | 1.158 | |
| Interaction KO/FL | 1.34E-03 | 1.14E-03 | 1.176 | Interaction KO/FL | 2.20E-03 | 9.64E-04 | 2.280 | * | |
| Interaction GBP/KO | −2.21E-03 | 1.14E-03 | −1.944 | Interaction GBP/KO | 1.78E-04 | 9.65E-04 | 0.185 | ||
| CUX1 MZ | CTIP2 MZ | ||||||||
| FL Injury | −2.79E-03 | 9.17E-04 | −3.047 | * | FL Injury | −2.56E-03 | 9.44E-04 | −2.708 | * |
| GBP treatment | −3.86E-05 | 8.48E-04 | −0.046 | GBP treatment | −2.08E-04 | 8.51E-04 | −0.244 | ||
| α2δ-1 KO | 1.19E-03 | 9.10E-04 | 1.309 | α2δ-1 KO | 7.44E-04 | 9.38E-04 | 0.794 | ||
| Interaction GBP/FL | 2.32E-03 | 1.04E-03 | 2.232 | * | Interaction GBP/FL | 2.60E-03 | 1.07E-03 | 2.431 | * |
| Interaction KO/FL | 1.42E-03 | 1.05E-03 | 1.361 | Interaction KO/FL | 1.96E-03 | 1.09E-03 | 1.800 | ||
| Interaction GBP/KO | −1.79E-03 | 1.05E-03 | −1.706 | Interaction GBP/KO | 1.20E-04 | 1.09E-03 | 0.110 | ||
| Superficial/deep layer area ratio | Synapse number | ||||||||
| FL Injury | 5.613 | 1.523 | 3.685 | * | FL Injury | 301.184 | 78.931 | 3.816 | * |
| GBP treatment | NA | NA | NA | GBP treatment | 4.534 | 77.715 | 0.058 | ||
| α2δ-1 KO | NA | NA | NA | α2δ-1 KO | −49.436 | 77.715 | −0.636 | ||
| Interaction GBP/FL | −3.877 | 1.413 | −2.743 | * | Interaction GBP/FL | −195.500 | 90.606 | −2.158 | * |
| Interaction KO/FL | −5.235 | 1.556 | −3.365 | * | Interaction KO/FL | −142.822 | 90.606 | −1.576 | |
| Interaction GBP/KO | 3.710 | 2.132 | 1.740 | Interaction GBP/KO | −28.255 | 90.634 | −0.312 | ||
| fEPSP % epileptiform | fEPSP area | ||||||||
| FL Injury | 0.447 | 0.131 | 3.409 | * | FL Injury | 22.953 | 6.458 | 3.554 | * |
| GBP treatment | −0.179 | 0.132 | −1.352 | GBP treatment | −1.940 | 6.553 | −0.296 | ||
| α2δ-1 KO | −0.177 | 0.140 | −1.265 | α2δ-1 KO | −7.069 | 6.365 | −1.111 | ||
| Interaction GBP/FL | −0.235 | 0.148 | −1.588 | Interaction GBP/FL | −22.600 | 7.851 | −2.879 | * | |
| Interaction KO/FL | −0.211 | 0.147 | −1.439 | Interaction KO/FL | −6.182 | 7.719 | −0.801 | ||
| Interaction GBP/KO | 0.283 | 0.147 | 1.926 | Interaction GBP/KO | 15.274 | 7.839 | 1.948 | ||
*t value > 1.96 or < −1.96.