Figure 1.

Development of CB-5083-resistant cells. (a) Experimental setup used to generate the initial OVSAHO-resistant cells. (b) Colony formation assay was performed on parental OVSAHO cells and OVSAHO-R (resistant) cells. Cells were treated with different concentrations of CB-5083 between 0.5–2.5 μM for 48 h and then allowed to recover under normal media for eight additional days. (c) Experimental setup used to generate O-CB-R1 and O-CB-R2 from OVSAHO-R. (d) OVSAHO parental, O-CB-R1 and O-CB-R2 were incubated with incremental doses of CB-5083 (0.1 –10 μM) for 72 h and cell viability was determined using SRB assay. Dose–response curves were generated via GraphPad Prism using four parameters nonlinear regression and the curves were constrained on top (100%) and bottom (>0%). Every point in the dose response curve represents Mean±S.E.M. taken from at least duplicate samples for all cell lines. (e) The bar graph represents Mean GI₅₀+S.E.M. taken from three independent experiments in all cell lines. P-values were calculated using Student’s t-test. (f) Images of OVSAHO parental, O-CB-R1 and O-CB-R2 were taken using a Zeiss light microscope at ×100 magnification. **P value⩽0.01.