Table 2.
Immunohistochemistry scores for I-FABP and relative abundance by gut segment. Score is histological intensity. Photometric correction is a multiplier that adjusts visual scoring intensity values to stepwise changes in concentrations quantified photometrically by a spectrophotometer. Square meters (m2) of mucosal surface area were obtained from Helander and Fändriks [15]. Duodenum tissue was not available for this study, so the score was estimated from Besnard et al. [16]. Size correction (size corr.) was used to allow for distribution across the same segment (e.g., corpus is approx. 70% of the stomach). Relative abundance is apparent relative abundance of I-FABP in stated segment (score × area × size corr.), whereas corrected relative abundance replaces score with photometric correction. The last two columns give abundances as percent of the total GI abundance.
| Segment | Score | Photometric correction | Area m2 | Size corr. | Relative abundance | Corr. rel. abundance | % abundance | Corr. % abundance |
|---|---|---|---|---|---|---|---|---|
| Cardia | 3 | 16 | 0.05 | 0.1 | 0.015 | 0.08 | 0.011 | 0.004 |
| Fundus | 2 | 4 | 0.05 | 0.2 | 0.02 | 0.04 | 0.015 | 0.002 |
| Corpus | 2 | 4 | 0.05 | 0.7 | 0.07 | 0.14 | 0.053 | 0.007 |
| Duodenum | 2 | 4 | 3.3 | 1.0 | 6.6 | 13.2 | 4.973 | 0.672 |
| Jejunum | 4 | 64 | 15.0 | 1.0 | 60.0 | 960.0 | 45.213 | 48.844 |
| Illeum | 4 | 64 | 15.0 | 1.0 | 60.0 | 960.0 | 45.213 | 48.844 |
| Colon | 3 | 16 | 2.0 | 1.0 | 6.0 | 32.0 | 4.521 | 1.628 |
| Sum | N/A | N/A | 35.45 | N/A | 132.705 | 1965.46 | 100 | 100 |