Table 1.
Comparison Between Plasma and Whole Blood in Multiplex Assays
| Analyte | Assay LOD | Units | Plasma range | Plasma mean | WB range | WB mean | p-Value |
|---|---|---|---|---|---|---|---|
| ADAMTS13* | 122 | ng/mL | 422–762 | 555 | 220–872 | 391 | 0.01 |
| CFH* | 4,883 | ng/mL | 176,801–276,624 | 225,882 | 80,983–211,069 | 161,698 | 0.0002 |
| CRP | 0.5 | mg/L | 0.5–28 | 7 | 0.5–17 | 5 | 0.08 |
| D-dimer* | 0.003 | μg/mL | 0.2–1.3 | 0.7 | 0.3–3.1 | 1.7 | 0.004 |
| E-selectin | 250 | pg/mL | 8,901–67,177 | 43,226 | 7,138–61,337 | 40,112 | 0.08 |
| EGF* | 18 | pg/mL | 18–288 | 71 | 33–354 | 90 | 0.03 |
| Eotaxin* | 10 | pg/mL | 24–59 | 40 | 27–140 | 68 | 0.03 |
| Ferritin* | 2 | ng/mL | 26–117 | 61 | 38–163 | 87 | 0.03 |
| FGF-basic | 12 | pg/mL | 17–851 | 135 | 23–967 | 150 | 0.3 |
| Fibrinogen* | 0.1 | mg/dL | 143–241 | 191 | 60–143 | 109 | <0.0001 |
| GROα* | 2 | pg/mL | 9–15 | 12 | 9–14 | 11 | 0.03 |
| HGF | 41 | pg/mL | 232–960 | 414 | 214–1,290 | 426 | 0.8 |
| IFN-α | 22 | pg/mL | 22–70 | 31 | 24–86 | 37 | 0.09 |
| IFN-γ* | 14 | pg/mL | 15–48 | 35 | 36–55 | 47 | 0.0003 |
| IL-12 (p40/p70)* | 21 | pg/mL | 54–120 | 90 | 32–91 | 61 | 0.0002 |
| IL-15 | 118 | pg/mL | 118–2,568 | 555 | 118–3,666 | 654 | 0.5 |
| IL-1RA* | 110 | pg/mL | 110–217 | 168 | 2,154–6,640 | 4,196 | 0.0002 |
| IL-29 (IFN-λ) | 57 | pg/mL | 111–144 | 125 | 100–122 | 111 | 0.1 |
| IL-2R | 64 | pg/mL | 178–382 | 255 | 168–323 | 240 | 0.3 |
| L-selectin* | 38 | pg/mL | 14,507–33,549 | 24,671 | 19,854–34,025 | 27,570 | 0.003 |
| MCP-1* | 76 | pg/mL | 138–443 | 243 | 138–543 | 383 | 0.01 |
| MCP-2* | 0.5 | pg/mL | 3–9 | 6 | 2–4 | 3 | 0.002 |
| MIP-1α | 50 | pg/mL | 50–175 | 77 | 50–248 | 84 | 0.3 |
| MIP-1β | 22 | pg/mL | 35–2,494 | 392 | 39–2,716 | 407 | 0.6 |
| P-selectin* | 1,233 | pg/mL | 9,510–78,642 | 24,796 | 15,273–68,604 | 45,232 | 0.02 |
| PAI-1* | 19 | pg/mL | 2,176–4,354 | 3,332 | 3,513–7,989 | 5,923 | 0.0006 |
| PECAM-1* | 407 | pg/mL | 12,601–28,591 | 22,029 | 13,393–22,612 | 18,124 | 0.002 |
| PF4* | 366 | ng/mL | 366–4,234 | 1,635 | 3,078–8,000 | 5,612 | 0.0001 |
| RANTES | 53 | pg/mL | 460–951 | 687 | 472–1,784 | 933 | 0.07 |
| TNF-R1* | 170 | pg/mL | 4,074–6,959 | 5,555 | 1,083–5,098 | 2,694 | 0.0006 |
| TNF-R2* | 1 | pg/mL | 93–247 | 166 | 85–196 | 132 | 0.04 |
| tPA | 39 | pg/mL | 244–572 | 389 | 230–405 | 347 | 0.3 |
| VCAM* | 1,890 | pg/mL | 73,511–308,212 | 164,569 | 43,797–117,551 | 78,364 | 0.01 |
| vWF | 2,436 | ng/mL | 3,981–11,923 | 6,748 | 3,638–9,791 | 6,525 | 0.8 |
Starred (*) analytes indicate a p-value <0.05 as determined by the paired Student's t-test.
LOD, limit of detection; IFN, interferon; IL, interleukin; GRO, growth-regulated protein; MIP, macrophage inflammatory protein; MCP, macrophage chemoattractant protein; RANTES, regulated upon activation, normal T cell expressed and secreted; CFH, complement factor H; CRP, c-reactive protein; TNF, tumor necrosis factor; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; PAI, plasminogen activator inhibitor; PF, platelet factor; tPA, tissue plasminogen activator; vWF, vonWillebrand factor; PE-CAM, platelet and endothelial cell adhesion molecule; VCAM, vascular cell adhesion molecule; EGF, epidermal growth factor; FGF, fibroblast growth factor; HGF, hepatocyte growth factor; ICAM, intracellular adhesion molecule, did have detectable levels in all samples but was excluded from this analysis due to insufficient bead counts.