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. Author manuscript; available in PMC: 2017 Nov 6.
Published in final edited form as: Nat Cell Biol. 2017 Apr 17;19(5):433–444. doi: 10.1038/ncb3505

Figure 6. Heterogeneous gene expression among FSCs.

Figure 6

(a–i) Expression of markers along the A/P axis; arrows indicate the border of Fas3 expression (green), layer 1 (white), 2 (cyan) and 3 (yellow) FSCs. (a–c) Castor (Cas, red in a, b, gray in c) stains FCs and all FSCs, while the stat-GFP (green) JAK-STAT pathway reporter also extends into region 2a ECs. (d) PZ1444- lacZ (red) and (g–i) the Fz3-RFP Wnt pathway reporter (red in g, i) show strong expression in ECs and weaker expression in FSCs, with reduced or undetectable expression in layer 1 FSCs (white arrows) and FCs (pink arrows); Fas3 (green). (h) shows Fz3-RFP (gray) from the same germarium as (g). (e,f) Fax-GFP (green in e, gray in f) is expressed more strongly in FSCs than in FCs or ECs. (i) Diagonal white line indicates an edge of the original image (shown in Supplementary Fig. 9). Scale bars 20μm.