Figure 5.

Circadian gene dynamics in human adult stem cells after rhythmic mechanical stimulation protocol. (a) Schematic diagram outlining the schedule of the 12 h ON : 12 h OFF (stretch : rest) regime followed by sample collection every 4 h between 16 h–52 h. (b) Quantitative RT-PCR analyses showing temporal expression profiles of clock genes following 3 days of rhythmic mechanical stimulation. Data are expressed as the mean +/− of ΔCt ± SEM relative to 16 h time point and normalised against the housekeeping gene GAPDH. Bars represent means ± SEM of 3 independent experiments.