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. Author manuscript; available in PMC: 2017 Nov 6.
Published in final edited form as: Anal Chem. 2016 Jun 27;88(14):7302–7311. doi: 10.1021/acs.analchem.6b01655

Table 2.

Beam Width Values Obtained from Edgespread Analysis Across Multiple Rising and Falling Edge Profiles of Known Spacings under Different Focus and Power Settingsa

laser focus setting laser power (μJ) average signal/ pixel (×104) σ (μm) fwhm (μm)
ultra 4.66 ± 0.17 9.0 16.8 ± 1.0 40 ± 2
4.66 ± 0.17 9.6 16.4 ± 0.8 38.6 ± 1.9
medium 10.0 ± 0.3 7.5 13.1 ± 0.7 30.8 ± 1.6
9.9 ± 0.3 0.3 12.2 ± 2.7 29 ± 6
small 4.69 ± 0.13 7.1 5.4 ± 0.9 13 ± 2
4.69 ± 0.13 8.0 5.5 ± 0.8 13.0 ± 1.9
a

Data were collected using a Bruker Ultraflex. The laser power as percentages were 82.0, 83.2, and 84.8% for ultra, medium, and small focus settings, respectively.