Table 2.
laser focus setting | laser power (μJ) | average signal/ pixel (×104) | σ (μm) | fwhm (μm) |
---|---|---|---|---|
ultra | 4.66 ± 0.17 | 9.0 | 16.8 ± 1.0 | 40 ± 2 |
4.66 ± 0.17 | 9.6 | 16.4 ± 0.8 | 38.6 ± 1.9 | |
medium | 10.0 ± 0.3 | 7.5 | 13.1 ± 0.7 | 30.8 ± 1.6 |
9.9 ± 0.3 | 0.3 | 12.2 ± 2.7 | 29 ± 6 | |
small | 4.69 ± 0.13 | 7.1 | 5.4 ± 0.9 | 13 ± 2 |
4.69 ± 0.13 | 8.0 | 5.5 ± 0.8 | 13.0 ± 1.9 |
Data were collected using a Bruker Ultraflex. The laser power as percentages were 82.0, 83.2, and 84.8% for ultra, medium, and small focus settings, respectively.