Figure 2.

Immunostaining of separase with established and experimental markers of proliferation in breast carcinoma. Double-IF stainings were performed for combinations of separase (green) with Ki-67, PHH3 and securin (red). In all double-IFs, separase showed co-expression in proliferating cells but the association was most clear between separase and PHH3. Double-IF for separase (red) and Cleaved casepase-3 (green) showed that separase was not expressed in apoptotic cells. (magnification × 400). A full colour version of this figure is available at the British Journal of Cancer journal online.