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. Author manuscript; available in PMC: 2018 Oct 11.
Published in final edited form as: J Am Chem Soc. 2017 Sep 27;139(40):14251–14256. doi: 10.1021/jacs.7b07798

Figure 4.

Figure 4

(a) Schematic illustration of orthogonal assembly of protein-functionalized particles. Red fluorescent particles are coated with Z17 and Z18, and green fluorescent particles are coated with SpyTag and SpyCatcher. (b) Orthogonal assembly in a 1:1:1:1 particle mixture of Z17 (red), Z18 (red), SpyCatcher (green) and SpyTag(green) in PBS with 0.005% tween 20 mixed at 25 °C for 40 min. (c) Selective dissociation of Z17–Z18 aggregates by 1 mg/mL soluble Z17 for 24 h. (d) Colocalization plot of red and green particles shown in (b).