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. 2017 Oct 23;51(6):1722–1730. doi: 10.3892/ijo.2017.4175

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Copyright: © He et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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The effects of CASC15 knockdown on tumor growth in vivo. (A) The CASC15 expression level was determined by reverse-transcription quantitative polymerase chain reaction after transfection of SMMC7721 cells with sh-CASC15. (B) Tumors from the nodes of mice 21 days after injection of SMMC7721 cells stably transfected with sh-CASC15 or empty vector. (C) The tumor volumes are expressed as means ± SD. (D) The tumor weights are expressed as means ± SD. (E) Representative image of immunohistochemical staining of the tumor. The sh-CASC15 group showed lower Ki-67 protein levels compared with the control. Left, hematoxylin and eosin staining. Right, immunostaining. The abbreviations are the same as in Fig. 1. ^*P<0.05, ^**P<0.01, ^***P<0.001. Magnification, ×100.