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. 2017 Oct 16;51(6):1639–1650. doi: 10.3892/ijo.2017.4165

Figure 1.

Figure 1

E2F1 silencing not only reduces osteosarcoma cell proliferation but attenuates migration and invasion. (A) The different expressions of E2F1 at the protein level in osteoblast (hFOB1.19) or osteosarcoma (U2OS and SaOs-2) cells were examined. (B) Shscramble and two E2F1 shRNAs were transfected into U2OS or SaOs-2 cells, and the protein levels of E2F1 were measured to indicate their efficacy. (C) Cell viability assay in U2OS or SaOs-2 cells stably transfected with shscramble and shE2F1. (D and E) Cell migration or invasion activity was examined by wound healing or Transwell assay. Representative images at 24 h are shown (left panels), and wound areas or invasive cells were quantified (right graph). Scale bar, 200 µm (wound healing); 100 µm (Transwell). (F) U2OS and SaOs-2 cells stably transfected as indicated, were subjected to cytometric analysis of apoptotic cells after staining with Annexin V/PI. (A-F) Data shown are mean ± SEM, each performed in triplicate. *P<0.05.