Figure 3.

Silence of DDR1 reduces the levels of p-AKT and p-STAT3 accompanied with an attenuated aggressiveness of osteosarcoma cells. (A) Shscramble and two DDR1 shRNAs were transfected into U2OS or SaOs-2 cells, and the protein levels of DDR1 were measured to indicate their efficacy. (B) U2OS or SaOs-2 cells were stably transfected with shscramble and shDDR1, and the expression of pAKT, total-AKT, pY705-STAT3, and total-STAT3 was examined. (C) Cell viability assay in U2OS or SaOs-2 cells stably transfected with shscramble and shDDR1. (D and E) Cell migration or invasion activity was examined by wound healing or Transwell assay. Wound areas or invasive cells were quantified. (A-E) Data shown are mean ± SEM, each performed in triplicate. ^*P<0.05.