Figure 4.

DDR1 mediates E2F1-dependent malignant phenotypes of osteosarcoma cells. (A) Shscramble or shE2F1-U2OS cells were transfected with empty vector and DDR1 plasmids, and the expression of pAKT, total-AKT, pY705-STAT3, and total-STAT3 was examined. (B) Cell viability assay in the indicated U2OS cells. (C and D) Cell migration or invasion activity was examined by wound healing or Transwell assay. Representative images at 24 h are shown (left panels), and wound areas or invasive cells were quantified (right graph). Scale bar, 200 µm (wound healing); 100 µm (Transwell). (B-D) Data shown are mean ± SEM, each performed in triplicate. ^*P<0.05.