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. 2017 Oct 20;51(6):1694–1704. doi: 10.3892/ijo.2017.4171

Figure 3.

Figure 3

SRC3 expressed in BMSCs is involved in the proliferation and migration of multiple myeloma cells. The RPMI-8226 cells were co-cultured with either BMSCs or sh-SRC3-BMSCs and their proliferation and migration ability were assessed. (A) Cell proliferation analysis of RPMI-8226 cells after co-culture for 48 h using CCK-8 assay. (B) Hoechst staining of co-cultured RPMI-8226 cells. (C) Cells positive for Hoechst staining were counted. (D and E) Scratch-wound healing assay assessed the migration ability of RPMI-8226 cells after being co-cultured for 48 h. The wound closure was calculated at 24 h under a phase contrast microscope. (F) Transwell migration assay was performed to test the change in migration ability of RPMI-8226 cells after being co-cultured for 48 h. (G) Quantitative assay of migrating cells under a phase contrast microscope. Data represent three independent experiments (average and SEM of triplicate samples). *P<0.05, **P<0.01 vs. control; ##P<0.01 vs. MM+sh-SRC3-MSC.