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. 2017 Oct 19;51(6):1661–1673. doi: 10.3892/ijo.2017.4167

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Copyright: © Dachineni et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Aspirin acetylates recombinant CDK1 in vitro and cellular CDK1 in HCT116 cells. (A) Upper panel, dose-dependent effect of aspirin on recombinant CDK1 acetylation. (B) Salicylic acid pre-incubation prevents aspirin-induced acetylation (upper panel). (C) Quantification of blot in (B), the decrease in CDK1 acetylation is expressed as a percentage of the control. (D) Pre-incubation of recombinant CDK1 with 2,3-DHBA and 2,6-DHBA at 0.5 mM prevents aspirin induced acetylation of CDK1 (upper panel). (E) Pre-incubation of recombinant CDK1 with 2,4,6-THBA prevents aspirin induced acetylation of CDK1 (upper panel). (F) Aspirin acetylates cellular CDK1 in HCT-116 cells (upper panel). (F) Lower panel, equal amounts of proteins was immunoblotted with anti-CDK1 antibody. The lower panels in all other blots (A, B, D and E), the upper panel blots were stripped and reprobed with anti-CDK1 antibody. **P<0.01, ^‡P<0.001.