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. 2017 Oct 19;51(6):1661–1673. doi: 10.3892/ijo.2017.4167

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Copyright: © Dachineni et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of aspirin, salicylic acid, 2,3-DHBA, 2,6-DHBA and 2,4,6-THBA (at 0.5 mM) on CDK2, 4 and 6 enzyme activity. (A) Upper panel represents in vitro kinase assays showing the effect of aspirin, salicylic acid, 2,3-DHBA and 2,6-DHBA on CDK2 activity. Lower panel shows coomassie stained H1 histones. (B) Quantification of the blot in (A). (C) Upper panel represents in vitro kinase assays showing the dose-dependent effect of 2,4,6-THBA on CDK2 activity. Lower panel shows coomassie stained H1 histones. (D) Quantification of the blot in (C). (E) Upper panel represents the effect of aspirin, salicylic acid, 2,3-DHBA and 2,6-DHBA and 2,4,6-THBA on CDK4 activity. Lower panel shows coomassie stained retinoblastoma protein. (F) Quantification of the blot in (E). (G) Upper panel represents the effect of aspirin, salicylic acid, 2,3-DHBA and 2,6-DHBA (0.5 mM) and 2,4,6-THBA on CDK6 enzyme activity. Lower panel shows coomassie stained retinoblastoma protein. (H) Quantification of the blot in (G). ^*P<0.05, ^**P<0.01, ^†P<0.001, ^‡P<0.001.

Effect of aspirin, salicylic acid, 2,3-DHBA, 2,6-DHBA and 2,4,6-THBA (at 0.5 mM) on CDK2, 4 and 6 enzyme activity. (A) Upper panel represents in vitro kinase assays showing the effect of aspirin, salicylic acid, 2,3-DHBA and 2,6-DHBA on CDK2 activity. Lower panel shows coomassie stained H1 histones. (B) Quantification of the blot in (A). (C) Upper panel represents in vitro kinase assays showing the dose-dependent effect of 2,4,6-THBA on CDK2 activity. Lower panel shows coomassie stained H1 histones. (D) Quantification of the blot in (C). (E) Upper panel represents the effect of aspirin, salicylic acid, 2,3-DHBA and 2,6-DHBA and 2,4,6-THBA on CDK4 activity. Lower panel shows coomassie stained retinoblastoma protein. (F) Quantification of the blot in (E). (G) Upper panel represents the effect of aspirin, salicylic acid, 2,3-DHBA and 2,6-DHBA (0.5 mM) and 2,4,6-THBA on CDK6 enzyme activity. Lower panel shows coomassie stained retinoblastoma protein. (H) Quantification of the blot in (G). ^*P<0.05, ^**P<0.01, ^†P<0.001, ^‡P<0.001.